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KMID : 1134820140430081166
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Journal of the Korean Society of Food Science and Nutrition
2014 Volume.43 No. 8 p.1166 ~ p.1173
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Protective Effects of Enzymatic Oyster Hydrolysate on Acetaminophen-induced HepG-2 Cell Damage
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Park Si-Hyang
Moon Sung-Sil
Xie Cheng-Liang
Choung Se-Young
Choi Yeung-Joon
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Abstract
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This study investigated the detoxification effects of enzymatic hydrolysate from oyster on acetaminophen-induced toxicity using HepG-2 cells. Oyster hydrolysate was made with 1% Protamex and 1% Neutrase after treatment with transglutaminase (TGPN) or without (PN). Two types of oyster hydrolysate were added to human-derived HepG-2 hepatocytes damaged by acetaminophen, after which the survival rate of HepG-2 cell was measured. In addition, glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) activities in the culture media were evaluated. The survival rates of HepG-2 cells were 136.2±1.4% at 100 μg/mL of TGPN and 179.6±3.8% at 200 μg/mL of TGPN. These cell survival rates were higher compared to that of the negative control group (60.7±3.2%) treated only with acetaminophen. GOT activity was 38.3±0.2 Karmen/mL in the negative control group, whereas it was 19.9±0.5 for TGPN (200 μg/mL) and 22.0±2.4 Karmen/mL for PN (200 μg/mL). GOT and GTP activities were shown to be dependent on TGPN concentration, and significant reduction in activities could be conformed. The detoxification efficacy of TGPN was higher compared to that of PN. These results suggest that oyster hydrolysate has potential as a healthy food or pro-drug for liver protection.
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KEYWORD
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oyster, hydrolysate, acetaminophen, detoxification of drug, peptide
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